Evaluation of Commercially Available Chikungunya Virus Immunoglobulin M Detection Assays
نویسندگان
چکیده
منابع مشابه
Evaluation of Commercially Available Chikungunya Virus Immunoglobulin M Detection Assays
Commercial chikungunya virus (CHIKV)-specific IgM detection kits were evaluated at the Centers for Disease Control and Prevention (CDC), the Public Health Agency of Canada National Microbiology Laboratory, and the Caribbean Public Health Agency (CARPHA). The Euroimmun Anti-CHIKV IgM ELISA kit had ≥ 95% concordance with all three reference laboratory results. The limit of detection for low CHIK ...
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Anti-dengue virus immunoglobulin M kits were evaluated. Test sensitivities were 21%-99% and specificities were 77%-98% compared with reference ELISAs. False-positive results were found for patients with malaria or past dengue infections. Three ELISAs showing strong agreement with reference ELISAs will be included in the World Health Organization Bulk Procurement Scheme.
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Chikungunya virus (CHIKV) is present or emerging in dengue virus-endemic areas. Infections caused by these viruses share some common signs/symptoms, but prognosis, patient care, and persistent symptoms differ. Thus, accurate diagnostic methods are essential for differentiating the infections. We evaluated 4 CHIKV serologic diagnostic tests, 2 of which showed poor sensitivity and specificity.
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In the recent paper of O’Connor et al. (1), four rapid methods for the detection of toxins of Clostridium difficile were evaluated. I have two main criticisms regarding the methodology of this study. First, for the different methods examined, the specimen treatment procedures were described as follows: for the culture procedure, “stool specimens were cultured on the day of receipt”; for the cyt...
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We assessed two two-step and two analog assays for measuring free thyroxin (FT4) in serum: Clinical Assays' "GammaCoat Free/Total T4" (CA), Vitek's "KinetiCount Phase II Free T4" (VTK), Diagnostic Products Corporation's "Coat-a-Count Free T4" (DPC) kit (June 1987 version), and Amersham's "Amerlex-M Free T4" (AMX). The VTK assay is automated except for the initial pipetting step. Interassay resu...
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ژورنال
عنوان ژورنال: The American Journal of Tropical Medicine and Hygiene
سال: 2016
ISSN: 0002-9637,1476-1645
DOI: 10.4269/ajtmh.16-0013